Membranes in lupin root nodules. I. The role of Golgi bodies in the biogenesis of infection threads and peribacteroid membranes.
نویسندگان
چکیده
The process of infection of lupin nodule cells by rhizobia was examined using thin-section and freeze-fracture electron-microscopic techniques to characterize the properties of different membranes and to establish relationships between them. The membranes of the Golgi bodies and the endoplasmic reticulum stained with zinc iodide-osmium tetroxide but not with phosphotungstic acid or silver. By contrast the infection thread membranes, peribacteroid membranes, plasma membranes and membranes of cytoplasmic vesicles did not stain with zinc iodide-osmium tetroxide but stained with phosphotungstic acid and silver. The peribacteroid membranes and plasma membranes are, however, different from each other since the particle density on the E face of freeze-fracture replicas of plasma membranes was twice that on the E face of the peribacteroid membranes. An examination of the tips of the infection threads in the cytoplasm of the plant cells, showed that the rhizobia bud off from the infection threads enclosed in the infection thread membranes. The rhizobia continue to divide still surrounded by membranes of plant origin, namely the peribacteroid membranes. Cytoplasmic vesicles are observed in both thin-section and freeze-fracture preparations of nodule tissue closely associated with, and apparently produced by, Golgi bodies. Formation of the walls and membranes of the infection threads and of the peribacteroid membranes involves fusion of the cytoplasmic vesicles with these membranes. It is proposed that the process of infection of plant cells in lupin nodules involves a change in the function of the Golgi body system for the biogenesis of plant cell walls and plasma membranes to include the synthesis of the walls and membranes of the infection threads and also the peribacteroid membranes.
منابع مشابه
Coated and smooth vesicles in the biogenesis of cell walls, plasma membranes, infection threads and peribacteroid membranes in root hairs and nodules of white clover.
متن کامل
Division of peribacteroid membranes in root nodules of white clover.
Division of peribacteroid membranes in the cytoplasm of root nodules of white clover was found, from a study of serial thin sections prepared for electron microscopy, to accompany division of the bacteroids. It was also observed that the peribacteroid membranes appeared to have adhered to various sites on the surface of the bacteroid envelope outer membranes. Wherever peribacteroid membranes we...
متن کاملPhospholipid Transfer from ER to the Peribacteroid Membrane in Soybean Nodules
Organelle membranes subfractionated from nodules o f Glycine max differed in lipid com po sition. Of the enzymes assembling phospholipids, choline kinase (EC 2.7.1.32) was recovered in the cytoplasmic fraction whereas CDP choline 1,2-diacylglyceride choline phosphotransferase (EC 2.7.8.2) was located in the ER. When methyl [14C]CDP choline was supplied to nodules in vivo radioactivity was foun...
متن کاملMolecular composition and development of the plant glycocalyx associated with the peribacteroid membrane of pea root nodules
In root nodules of pea (Pisum sativum), endosymbiotic Rhizobium bacteroids are individually enclosed by a plant-derived membrane, the peribacteroid membrane, creating an organelle-like structure termed the symbiosome. In order to investigate the structure and function of the peribacteroid membrane in plant-microbe surface interactions, monoclonal antibodies were used to identify the major antig...
متن کاملEffects of Boron on Rhizobium-Legume Cell-Surface Interactions and Nodule Development.
Boron (B) is an essential micronutrient for the development of nitrogen-fixing root nodules in pea (Pisum sativum). By using monoclonal antibodies that recognize specific glycoconjugate components implicated in legume root-nodule development, we investigated the effects of low B on the formation of infection threads and the colonization of pea nodules by Rhizobium leguminosarum bv viciae. In B-...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Journal of cell science
دوره 30 شماره
صفحات -
تاریخ انتشار 1978